presto blue reagent (Thermo Fisher)

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Thermo Fisher presto blue reagent
Presto Blue Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/presto+blue+reagent/Phenol+Red/10__1021_slash_acsorginorgau__5c00107-163-19-28
Average 99 stars, based on 1 article reviews

presto blue reagent - by Bioz Stars, 2026-10

99/100 stars

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Incubation:

Article Title: Aggregation Tendency, Cellular Uptake, and Viability Effects of Structurally Distinct Carbazole–Phthalocyanine Gold Nanoconjugates
Article Snippet: .. Following the 24 h incubation, the media were removed, the cells were washed with prewarmed PBS, and then 10% Presto Blue reagent (in DMEM without phenol red (#31053028 Gibco, USA)) was applied to all wells. .. After 1.5 h of incubation, the optical density was measured at 570 and 600 nm using a multimode plate reader (Victor Nivo 5T, PerkinElmer USA).

Article Title: Potentiation of Gelonin Cytotoxicity by Pulsed Electric Fields.
Article Snippet: .. At 23 h after pulse treatments, 10 μL of the Presto Blue reagent (Thermo Fisher Scientific, Richmond, VA, USA) was added to each well and incubation continued for 1 h at 37 ◦C. .. The plates were read with a Synergy 2 microplate reader (BioTEK, Winooski, VT, USA), with ex./em. settings at 530/590 nm.

Article Title: Low concentration press cake protein isolates preserve biological activity during lyophilization and spray drying
Article Snippet: .. Shortly, 10 μL Presto Blue reagent (ThermoFisher #A13262) was added to the remaining 90 μL of medium in each well before incubation at 37°C for 1 h. After incubation, the medium was transferred to a fresh 96-well plate (Greiner #655101) and the fluorescence at 560 nm (excitation) and 590 nm (emission) was measured with a plate reader (BioTek SynergyMx). .. For calculation of confluency, cells were seeded and treated the same way as described above for the short term proliferation experiments, but using Corning glass-bottom imaging plates (#CLS4580).

Article Title: Low-cost food-grade alternatives for serum albumins in FBS-free cell culture media.
Article Snippet: .. Shortly, 10 μL Presto Blue reagent (ThermoFisher #A13262) was added to the remaining 90 μl of medium in each well and incubated at 37 °C for 1 h. After incubation, the medium was transferred to a fresh 96-well plate (Greiner #655101) and the fluorescence at 560 nm (excitation) and 590 nm (emission) was measured with a plate reader (BioTek SynergyMx). ..

Fluorescence:

Cell Counting:

Article Title: PPARα-mediated lipid metabolism reprogramming supports anti-EGFR therapy resistance in head and neck squamous cell carcinoma
Article Snippet: In some conditions, cells were maintained in a medium supplemented with 10% delipidated FBS (Biowest) in presence or not of 1% chemically defined lipid concentrate (Thermo Fisher Scientific) or BSA-conjugated oleic and palmitic acids (Larodan). .. Cell growth was assessed either by direct cell counting on a hemocytometer with Trypan Blue exclusion dye or by using the Presto Blue reagent (Thermo Fisher Scientific) according to manufacturer’s instructions. .. For immunoblotting experiments, HNSCC cells were serum-starved for 4 h before incubation with 1 μg/mL cetuximab for 2 h and 20 nM epidermal growth factor (# 78006.1; STEMCELL Technologies) for 15 min.

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